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Twelve metabolites of a artificial cannabinoid, EAM-2201, ended up tentatively determined in human hepatocytes in vitro, and in human liver and urine specimens in vivo for the first time. There was a definite variance in metabolism profile involving the in vitro and in vivo outcomes.
EAM-2201 was incubated with human hepatocytes for three h in the CO2 incubator as well as the metabolites of EAM-2201 were being generated. The human liver specimen was homogenized along with the metabolites were being extracted. The urine specimen was hydrolyzed to start with with β-glucuronidase along with the metabolites were extracted.
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Then, the inhibitor concentration that supports 50 % the maximal level of inhibition (Ki) and maximal price of enzyme inhibition (kinact) values were being calculated employing the following equation with Enzyme Kinetics software:
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